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Isolation, characterization and cross-species amplification of polymorphic microsatellite markers for Oxytenanthera abyssinica (A. Rich.) Munro (Poaceae)

We developed microsatellite to investigate genetic diversity of Oxytenanthera abyssinica (Bindura bamboo) a bamboo species endemic to Senegal. Using the microsatellite-enriched library method, we identified 14 primer pairs. The primers amplified 10 di-nucleotide repeats and 4 composite motifs, the number of alleles ranged from 5 to 28 with an average of 11.9. The results indicate that these primer pairs offer an appropriate amount of variation to address issues of germplasm characterization, patterns of gene flow and conservation studies. Cross amplification has been tested in other bamboo species

Auteur(s) : ?Aliou Ndiaye, Ronan Rivallan, Thierry Legavre, Dominique Brunel, Maurice Sagna, Yaye Kène Gassama & Ange Marie Risterucci
Pages : 799-802
Année de publication : 2013
Revue : Conservation Genetics Resources
N° de volume : Volume 5 Number 3
Type : Article
Statut Editorial : Revue scientifique à comité de lecture, facteur d'impact 1.14
Mise en ligne par : NDIAYE Aliou