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Isolation and characterization of polymorphic microsatellites in the rodent Gerbillus nigeriae.

The new generation 454 FLX Titanium pyrosequencing technology was used to isolate microsatellite loci in the rodent Gerbillus nigeriae, a species which distribution area increasedrecently in sub-saharan Africa. Seven hundred and thirty two loci containing microsatellite sequences were obtained. From these, a total of 80 were tested in order to retain 12polymorphic markers that may be genotyped in two panels. Polymorphism was studied in two populations, one from Niger, and one from a recently invaded area in Senegal. Alleli diversity ranged from 2 to 13 alleles in Niger, and from 2 to 11 alleles in Senegal. These microsatellite markers will be useful to describe the invasion pattern of this species, associated with major climatic and environmental changes in Sahelian areas.


Auteur(s) : M. Thiam, K. Hima, P. Gauthier, C. Tatard, J.M. Duplantier, G. Dobogny, L. Granjon, A. Dalecky, K. Ba, M. Sembene and C. Brouat.
Pages : Database 1 October 2010 – 30 November 2010
Année de publication : 2011
Revue : Molecular Ecology Ressources 11: 418- 421. In Permanent Genetic Ressources added to Molecular Ecology Ressources
Type : Article
Mise en ligne par : THIAM Massamba