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Analysis of the Colorimetric Assay Technique of Serum Electrolytes (Sodium, Potassium)

Because the determination of electrolytes is important and adamant, reliable and rapid assay techniques must be available in every laboratory. Thus this work was conducted at the Laboratory of Biochemistry and Molecular Biology based at the University Cheikh Anta Diop of Dakar on a UVVisible spectrophotometer, to evaluate the analytical performance of the colorimetric assay technique of serum electrolytes (sodium, potassium) namely the detection limit, linearity, accuracy and appropriateness of measures in view to expand it nationwide. The results show a low detection limit, good linearity for the sodium, non-repeatability and non-reproducibility for both analysts and biases relatively and slightly higher compared to the values of the SFBC. Despite good detection limit, this colorimetric assay technique cannot be validated by the laboratory due to its instability.

Auteur(s) : S. Thiam1*, F. D. Agne1, O. Boye1, A. Samba1, F. Cissé1, A. Ndiaye1, D. Doupa2, I. Y. Soumah1, A. Diatta3, N. D. Sall1 and M. Touré
Pages : 1-9
Année de publication : 2016
Revue : Asian Journal of Biochemical and Pharmaceutical Research
N° de volume : 6 (3)
Type : Article
Mise en ligne par : SAMBA Abdourahmane