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New Spectrofluorimetric Method for Determining Serotonin : Application to Human Urine

In this present paper, we investigate the development of simple, rapid, accurate, reproducible and sensitive methods for the determination of serotonin (5-HT) in urine. For this purpose stationary fluorescence was used as method. With regard to the analysis of serotonin, the liquid-liquid extraction (LLE) solid phase extraction (SPE) and standard addition procedures were used. Several physicochemical factors affecting the sensitivity of the fluorescence intensity of serotonin were optimized, including the system of solvent (organic, micellar), the pH and the salts. The study of the analytical performances of the method led to very low limits of detection (LOD) varying between 0.1 and 3 ng/mL and to limits of quantification (LOQ) ranging between 0.4 and 10 ng/mL. This confirms the sensitivity of the method. Thus the low values of standard deviations (DRS) (between 0.3 and 6.6%) testify the good reproducibility of the measurements with satisfactory covering rate (89 to 111%). Accordingly, our results show that the spectrofluorimetric method is simple, fast and sensitive and can be applied to the routine analysis and does not require expensive equipment nor tiresome chemical pretreatments.

Auteur(s) : Abdourahmane Khonté, Diène Diégane Thiaré, Cheikh Diop, Lamine Cissé, François Delattre, Atanasse Coly, Mame Diabou Gaye-Seye, Niama Diop Sall, Alphon
Pages : 85-98
Année de publication : 2015
Revue : International Journal of Chemistry
N° de volume : (2) 7
Type : Article
Mise en ligne par : CISSE Lamine