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Acute, Sub-Chronic Toxicity in Wistar Rats and Cytotoxicity Studies of Hydroethanolic Root Extract of Cassia Sieberiana DC.

Acute and sub-chronic toxicity studies of hydroethanolic root extract of Cassia sieberiana (Caesalpiniaceae), a plant widely used in traditional medicine in Senegal, were carried out in Wistar rats. Cytotoxicity of this extract was studied on vervet monkey kidney cells by the MMT [3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide] method concurrently. In the acute toxicity study, five female rats (260 ± 10 g) were each administered a single oral dose (5000 mg/kg) of the hydroethanolic extract of C. Sieberiana root bark and observed for signs of acute toxicity for 14 days. In the sub-chronic toxicity study, the hydroethanolic extract at 500, 1000 and 2000 mg/kg was administered to sets of 6 Wistar rats daily for 28 days. Each set consisted of 3 males (278 ± 13g) and 3 females (260 ± 10 g). Urinalysis, haematological and biochemical analyses were carried out on urine, whole blood and serum samples. Histological studies of the liver, heart, and kidney tissues were also performed. The results showed that the acute and subchronic toxicity studies of hydroalcoholic root extract of C. sieberiana did not result in death among the animals; there were no signs of toxicity. However, the histological study showed the presence of necrotic and inflammatory cells in the hepatic parenchyma and a sinusoidal stasis at the end of 28 days. The cytotoxicity study on cell viability showed that the plant seems to be cytotoxic only at very high doses.


Auteur(s) : BAH F, KANE Y, FALL AD, CABRAL M, TOURE A, LAM-FAYE, DIOP C, DIENG SIM, DIOUF A and FALL.
Pages : p17
Année de publication : 2017
Revue : Journal of Toxicology and Pharmacology
N° de volume : 1 (3)
Type : Article
Mise en ligne par : TOURE Aminata