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Development and Validation of a Spectrofluorimetric Method for the Assay of Tetracycline in Capsules

The purpose of this study is to develop and validate a method for the analysis of tetracycline capsules by spectrofluorimetry. A pH 9 borate buffer was used as diluent of tetracycline after reaction with magnesium salt at the excitation wavelength of 372 nm and 516 nm of emission. A linear response was ob- served between 0.25 ?g/mL and 1.5 ?g/mL with a correlation coefficient (R) of 0.9998. The detection and quantification limits found are 0.0125 ?g/mL and 0.0412 ?g/mL respectively. The proposed method proved trueness with a re- covery between 99.88% and 101.10%. The relative standard deviations of re- peatability and intermediate precision found ?2.88% reflected a good preci- sion of the method. The proposed method is therefore valid within the limits of 90% to 110%. The proposed method was applied to the quality control of 9 tetracycline samples from market and gave results in accordance with the pharmacopoeia standards.


Auteur(s) : NAMEGABE LM, SARR SO, DIOP YM
Pages : 2018
Année de publication : 2018
Revue : American Journal of Analytical Chemistry
N° de volume : 9
Type : Article
Mise en ligne par : SARR Serigne Omar