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B subunits of cholera toxin and thermolabile enterotoxin of E. coli have similar adjuvant effect as whole molecules on rotavirus 2/6-VLP specific antibody responses and induce a Th17-like response after intrarectal immunization

The purpose of this study was to evaluate the adjuvant effect of the B subunits of cholera toxin (CT) and the thermolabile enterotoxin of Escherichia coli (LT) by the intrarectal route of immunization and compare them to the whole molecules CT and LT-R192G, a non toxic mutant of LT, using 2/6-VLP as an antigen, in mice. All molecules induced similar antigen specific antibody titers in serum and feces, whereas different T cell profiles were observed. CTB and LTB, conversely to CT and LT-R192G, did not induce detectable production of IL-2 by antigen specific T cells. Moreover, CTB, conversely to LT-R192G, CT and LTB, did not induce antigen specific CD4þCD25þFoxp3_ and Foxp3þ T cells, thus showing different effects between the B subunits themselves. However, all molecules induced an antigen specific Th17 response. In conclusion, B subunits are potent adjuvants on B cell responses by the intrarectal route. Although their impact on T cell responses are different, all molecules induce a 2/6-VLP-specific Th17 T cell response that may play a major role in helping B cell responses and thus in adjuvanticity and protection


Auteur(s) : Fatou Thiam, Annie Charpilienne, Didier Poncet, Evelyne Kohli, Christelle Basset
Pages : 27-34
Année de publication : 2015
Revue : Microbial Pathogenesis
N° de volume : 89
Type : Article
Statut Editorial : Elsevier (www.elsevier.com/locate/micpath
Mise en ligne par : THIAM Fatou