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Molecular characterization of group A rotaviruses circulating in Senegal over a 7-year period (2005-2011).

Introduction : Group A rotavirus is the most common cause of gastroenteritis among infants and young children. They are characterized by a genomic diversity and can be analyzed by polyacrylamide gel electrophoresis (PAGE) and/or by determination of the genic specificities with the VP7 glycoprotein defining G-types and the protease-sensitive VP4 defining P-types. The aim of this study is to follow the evolution of the group A Rotavirus strains circulating in Senegal over a 7- years period of (2005-2011). Materials and Methods: Positive Tool’s specimens by ELISA were analyzed by PAGE and were genotyped by Multiplex Hemi-Nested RT-PCR. Data were analyzed by Epi Info 7. Results: The rotavirus was found in 222 samples of tools among which 32 (14,41 %) were positive in PAGE. Five electropherotype pattern were highlighted with 23 long electrophoretypes and 9 short electrophoretypes. One hundred strains (45, 04 %) were identified with VP7 specificities highlighting five different genotypes: G1, G2, G3, G8 and G9. Also, 93 (41,89 %) were identified with VP4 specificities showing four different genotypes: P [4], P[6], P[8] and P[11]. Seventy six (34, 23 %) samples were genotyped simultaneously in VP7 and VP4, showing that the G1P [8] association prevails in Senegal followed by the G9 [ P8] association. Conclusion: There is a genetic diversity among rotavirus strains circulating in Senegal. Genotypes G1 [P8] and G9P [8] are predominant. We also finded mixed infections, which could favor the emergence of new viral strains, motivating a regular surveillance of genotypes circulating in the country, especially in the optics of the choice of a vaccine against this virus.

Auteur(s) : A. Diop, M. L. Dia, M. A. Sonko, D. Diop, R. Ka, A. I. Sow, M. Ba and M. F. Cissé.
Pages : 945-951
Année de publication : 2015
Revue : African Journal of Microbiology Research
N° de volume : 9(13)
Type : Article
Mise en ligne par : DIOP Amadou