HLADH-catalyzed synthesis of ?-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor
This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of
nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the ?-alanine.
Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory
conversions ( > 80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold
anode. An original method was proposed to limit the complexation effect of the produced ?-alanine to the
enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and
Michaelis constants was proposed.
Auteur(s) : R. A. Rodríguez-Hinestrozaa , C. Lópeza , J. López-Santína, C., Kane, , M. Dolors Benaigesa , T. Tzedakis
Pages : 196-207
Année de publication : 2017
Revue : Chem. Eng. Science
N° de volume : 158
Type : Article
Mise en ligne par : KANE Cheikhou