Prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae isolates in a Dakar university hospital, Senegal, 2013.
Introduction. - We had for objective to assess the prevalence of extended-spectrum ?-lactamase (ESBL)-producing isolates of Escherichia coli and Klebsiella pneumoniae and its antibiotic resistance patterns, because of their increase in our teaching hospital.
Material and methods. - Two hundred and seventy six E. coli and 198 K. pneumoniae isolates were investigated. Antimicrobial susceptibility testing was performed using the disk diffusion method. ESBL detection was performed using a double disc diffusion method.
Results. - ESBL were detected among 186 (39.2%) of all isolates. Frequency of ESBL production was 31.2% and 50.5% for E. coli and K. pneumoniae, respectively (p < 0.001). These ESBL strains were mainly isolated from patients hospitalized in intensive care units (ICU: 36.6%), particularly from urines (50.5%). All ESBL isolates were resistant to amoxicillin, cefalotine, cefamandole, aztreonam, and ceftazidim. Most of these ESBL strains had associated high resistance rates to non- ?-lactam antibiotics, including sulfamethoxazole/trimethoprim complex (90.5%), ciprofloxacine (78.7%), gentamicin (69.8%), and tetracycline (67.1). However, imipenem and fosfomycin remain very active with respectively 94.1% and 95.5 % of the ESBL strains susceptible.
Conclusion. - Resistance of E. coli and K. pneumoniae to antibiotics through the mechanism of ESBL production is becoming increasingly worrying. This suggests a more rational use of antibiotics, especially as first-time treatment of nosocomial infections.
Auteur(s) : Camara M, Ba-Diallo A, Diop-Ndiaye H, Karam F, Lo-Lo S, Diagne-Samb A, Toure-Kane NC, Mboup S, Gaye-Diallo A
Pages : 15-21
Année de publication : 2016
Revue : Rev Afric Biol Med
N° de volume : 1(1)
Type : Article
Mise en ligne par : CAMARA Makhtar