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Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic Babies.

The aim of the study was to assess T cell di?erentiation and the modulation of in?ammatory cytokines in obese and gestational diabetes mellitus (GDM) women and their macrosomic newborns. Hence, immediately after delivery, blood samples were collected through the mother’s arm vein and the umbilical cordon vein. Biochemical parameters measured were HbA1C, glucose, insulin, triglyceride (TG), total cholesterol (Tchol), HDL cholesterol (HDLchol), and LDL cholesterol (LDLchol). T lymphocytes were puri?ed from the total blood with Ficoll-Paque. The mRNA expression of in?ammatory markers in T cells was determined by RT-qPCR. We observed that diabetic mothers exhibited higher HbA1C, glycemia, insulinemia, TG, Tchol, HDLchol, and LDLchol levels than control mothers. Glycemia was not signi?cantly di?erent between macrosomic and control newborns. However, insulinemia was high in macrosomic babies. TG, Tchol, HDLchol, and LDLchol were not signi?cantly di?erent between macrosomic and control babies. In diabetic mothers, mRNA expression of the Th1 cell subtype was signi?cantly increased. Th1 markers were upregulated in babies born to diabetic women than in control newborns. However, expression of two Th2 markers (GATA3 and IL-4) was not signi?cantly di?erent between control and GDM women and between their respective newborns. Interestingly, IL-10 mRNA expression was decreased in diabetic mothers and their o?springs. The Th1/Th2 cytokine ratio was increased in GDM obese mothers and their macrosomic newborns, suggesting a proin?ammatory status in these subjects.


Auteur(s) : SECK A., HICHAMI A., DOUCOURE S., DIALLO AGNE F., BASSENE H., BA A., SOKHNA C., KHAN NA. AND SAMB A.
Pages : Article ID 8474617, 7 pages
Année de publication : 2018
Revue : Hindawi Journal of Diabetes Research
N° de volume : 2018-Article ID 8474617, 7 pages
Type : Article
Mise en ligne par : SECK Aïssatou